미생실 Differential medium

https://quizlet.com/4675318/microbio-225-lab-test-3-selective-and-differential-media-imvic-biochem-tests-with-some-bacterial-samples-flash-cards/

http://learn.chm.msu.edu/vibl/content/differential/index.html

미생실

http://study.com/academy/lesson/serial-dilution-in-microbiology-calculation-method-technique.html

http://www.bio-link.org/home/sites/files/instructor_manualv8.pdf

http://www.genehunters.co.kr/Training/01_2_2.htm
Competent Cell의 종류는 정말 많습니다.
E.coli가 가지는 유전자형에 따라서, 용도에 따라서, Transformation 방법에 따라서 나뉘어지죠. 여기서는 가장 보편적으로 이용하는 competent E. coli strain의 종류 및 특성에 대해서 간단히 비교 분석을 하겠습니다.

③ XL1-Blue competent E.coli
* 재조합 plasmid를 위한 blue-white color screening에 이용하며 또한 일반적인 plasmid 또는 lamda vector로 cloning할 때 사용한다.
* Genotype of E.coli strain XL1-Blue
*- tetracycline resistant
*- endonuclease(endA)deficient -> DNA miniprep시 질적인 향상.
*- recombinantion (recA)deficient -> Insert DNA의 안정성을 증가
*- LsdR mutation -> EcoK endonuclease에 의한 cloned DNA가 잘리는 것을 방지.
* IPTG and X-gal color screening 가능 (F’ episome 안에 lacIqZΔM15 gene으로 인해 가능)
* 통상적으로 45초가 heat shock을 시행한다.

분석화학 Titration methods

Displacement titration

A titration in which the analyte displaces a species, usually from a complex, and the amount of the displaced species is determined by a titration.  [c.275]

A second ligand in a complexation titration that initially binds with the analyte but is displaced by the titrant.  [c.316]

Ca + if a small amount of Mg -EDTA is added to the solution containing the analyte. The Mg + is displaced from the EDTA by Ca +, freeing the Mg + to form the red Mg -indicator complex. After all the Ca + has been titrated, Mg + is displaced from the Mg -indicator complex by EDTA, signaling the end point by the presence of the uncomplexed indicator s blue form.  [c.324]

http://chempedia.info/info/40168/

---

Stoichiometry > Back titration

The technique of back titration is used when the unknown compound cannot be dissolved in water for normal titration. The compound can however react with an acid, neutralising some of it. The remaining acid may then be titrated in the usual manner.

http://www.ibchem.com/IB16/03.55.htm

미생실 (3) Catalase test

http://learn.chm.msu.edu/vibl/content/catalase.html

 

The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme, catalase, is produced by bacteria that respire using oxygen, and protects them from the toxic by-products of oxygen metabolism. Catalase-positive bacteria include strict aerobes as well as facultative anaerobes, although they all have the ability to respire using oxygen as a terminal electron acceptor. Catalase-negative bacteria may be anaerobes, or they may be facultative anaerobes that only ferment and do not respire using oxygen as a terminal electron acceptor (ie. Streptococci).

헷갈렸던 유효숫자 계산

* (3.26x10^-3) – (7.88x10^-5) = (3.26x10^-3) - (0.0788x10^-3) = (3.182x10^-3) = (3.18x10^-3)
→ 뺄셈의 경우이므로 우선 같은 지수를 가지도록 맞춘 후(10^-3으로) 둘을 빼고, 가장 작은 소숫점상의 유효숫자가 2개이므로 결과도 소숫점상의 유효숫자가 2개가 되도록 맞춘다. 지수 두개를 늘리려면 소숫점을 오른쪽으로 두칸 움직여준다.

http://frontjang.info/286

ⓔ 567.000 - 12 = 555

→ 555

  12 소숫점 아래 x

 

ⓕ 8597 - 0.1 = 8596.9

→ 8597

  8597 소숫점 아래 x

 

ⓖ (3.50×10^5) × [2.8 / (5.4 – 4.09)] = 748091.60305...

5.4 – 4.09 = 1.31

→ 1.3

(3.50×10^5) × [2.8 / 1.3] = 753846.153846...

→ 750000 or 7.5×10^5

혼합계산은 계산 순서 대로! 괄호 안부터, 곱셈 나눗셈 부터
유효숫자는 2개여야

 

ⓗ (6.10×10^7) + (3×10^7)

= (6.10 + 3)×10^7 = 9.1×10^7

→ 9×10^7

 덧셈 계산. 같은 지수를 가지도록!

 

ⓘ 787 × 3.0 = 2361

→ 2400

 유효숫자는 2개여야
 

http://ywpop.tistory.com/4874